5. REAGENTS.
BASIC ELECTROPHORESIS GELS KIT
The kit includes all the reagents you need for making 10 full electrophoresis and the DNA visualization (for 10×7 cm o 10×10 cm gels).
2 formats:
* Ekitblue: 10 full electrophoresis.
> Kit includes: 10 agarose pills, 150 ml 10x TAE, 500 µl loading buffer, 25 ml DANABLUE, 100 ml FLASHBLUE.
EKITBLUE
* Ekitsafe: 10 full electrophoresis.
> Kit includes: 10 agarose pills, 150 ml 10X TAE, 500 µl loading buffer, 25 µl GELSAFE Nucleic Acid Gel Stain (UV light transilluminator needed).
EKITSAFE
DANABLUE-FLASHBLUE
NON TOXIC alternative for the nucleic acids detection. Nucleic acids are blue as electrophoresis occurs. UV light transilluminator is no needed to visualize correctly the DNA, but it requires a post-staining with FlashBlue.
> Kit includes: 25 ml DANABLUE, 100 ml FlashBlue.
DNB-100
GELSAFE Nucleic Acid Gel Stain
NON TOXIC alternative to the traditional ethidium bromide (EtBr) stain for detecting nucleic acid in agarose gels and with the same sensitivity. It needs a UV light transilluminator.
> Kit includes: 1 ml GELSAFE.
GELSAFE
AGAROSE POWDER
• 100 gr agarose powder: DANAGAROSE100
• 500 gr agarose powder: DANAGAROSE500
10x TAE
Agarose electrophoresis buffer for nucleic acids, the working concentration is 1x TAE.
• 10x TAE: 1 liter TAE1L
• 10x TAE: 500 ml TAE500ML
DANAPOLIMERASA HOT START
HOT START Polymerase
HOT START Polymerase (2x) ready for use, it allows amplify any fragment so that the user only has to add water and primers. An activation step of 10 minutes at 95ºC is required, in this way non-specific products as “primer-dimers” are removed. It also contains a red dye that allows direct seeding in the agarose gel without adding loading buffer.
> Kit includes: 1.25 ml POLYMERASE MIX.
1101
DANAMARKER BEETHOVEN
It contains 14 regularly spaced bands of 10,000 bp to 200 bp. Each band represents an exact amount of DNA, it allows DNA quantification. It is supplied in a format “ready to use”, using 20 µl in each load.
> Kit includes: 1 ml.
0405
DANAMARKER SCHUMANN
It contains 11 regularly spaced bands of 1,250 bp to 100 bp. Each band represents an exact amount of DNA, it allows DNA quantification. It is supplied in a format “ready to use”, using 20 µl in each load.
> Kit includes: 1 ml.
0406
DANAMARKER DEBUSSY
It is a mixture of 12 pre-stained proteins carefully selected to facilitate estimating molecular weight of the protein during SDS-polyacrylamide electrophoresis gel. This marker protein is ready to use and does not require previous preparations before being applied into the wells of a polyacrylamide gel.
> Kit includes: 500 µl.