1.1. EXTRACTION AND PURIFICATION OF
NUCLEICS ACIDS
BASIC LEVEL
DANAEXTRACTOR KIT
This practice is an introduction to molecular biology. This kit provides basic DNA extractions from animal tissues (chicken liver) and vegetables.
The kit includes everything you need so that each student can carry out an individual practice, in the classroom itself.
> Any additional equipment or material is not required.
INTERMEDIATE LEVEL
DANAEXTRACTOR SALIVA 2 Kit
This kit allows students make an extraction of their own DNA quickly and easily from a small sample of his saliva, a laboratory microcentrifuge is not needed.
To carry out this practice the laboratory has to have 20-200 microliters micropipettes and 100-1000 microliters micropipettes and their respective tips. This material also can be supplied by BIOTED.
The remaining material necessary to carry out the practice by each student individually is included in the kit.
The DNA appears as a strand in the solution that the student could take home.
DNES2-25 
25 students
ADVANCED LEVEL
The extraction process involves cell lysis to release nucleic acids and the inactivation of cellular nucleases (DNases and RNases) to prevent degradation of nucleic acids.
The purification process is the separation of the nucleic acids from the other components.
There are different techniques for purification:
A. SALTING-OUT
Proteins precipitation with high salt concentrations.
DANAGENE SALIVA KIT
This kit allows you to isolate DNA from a saliva sample of students to obtain a quality DNA for use in PCR.
> Kit includes:
Lysis solution, protein precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, isopropanol and 70% ethanol.
DANAGENE BLOOD DNA KIT
This kit allows you to isolate DNA from a blood sample to obtain a quality DNA for use in PCR.
> Kit includes:
RBC solution, lysis solution, proteins precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, isopropanol and 70% ethanol.
100 ml/blood
DANAGENE PLANT DNA KIT
This kit allows you to isolate DNA from different plants samples to obtain a quality DNA for use in PCR.
> Kit includes:
Extraction buffer, lysis solution, RNAse, PVP solution, protein precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, isopropanol and 70% ethanol.
B. ADSORPTION CHROMATOGRAPHY.
It is based on the adsorption capacity of nucleic acids to the silica membrane in the presence of chaotropic salts which come in small columns. We have a wide range of kits that use columns, you can visit at www.danagen.es.
We propose the following kits at educational level:
DANAGENE SPIN SALIVA DNA KIT
1.DANASALIVA Sample Collection Kit provides a safe and rapid all-in-one procedure for the collection, stabilization and transport of 2 ml saliva samples at room temperature that stabilizes DNA from the time of collection for several months.
Saliva samples are collected by spitting inside the collection funnel which has been assembled with the collection tube.After collecting 2 ml saliva, the contents of saliva preservation solution are added and mixed with the collected saliva. The saliva collection tube is sent to the laboratory for DNA isolation and analysis using the DANAGENE SALIVA DNA Kit o DANAGENE SPIN Saliva DNA Kit.
2.DANAGENE SPIN SALIVA DNA Kit has been designed for rapid and efficient purification of total DNA from saliva samples preserved with the DANASALIVA Sample Collection Kit. Briefly, 400 µl of preserved saliva is processed by lysing the cells with Lysis Buffer and Proteinase K by incubation at 55ºC for 20 minutes. After centrifugation, the binding conditions to the column membrane are adjusted by adding ethanol and the lysate is added to the MicroSPin column. The DNA bound to the column is subjected to 2 washing steps to remove inhibitors. After a drying step, the DNA can be eluted with 50 µl Elution Buffer5 mM Tris/HCl pH 8.5.
0603.43EDU 
1 STUDENT (COLLECTION KIT)
0603.SPIN50 50 STUDENTS
0603.SPIN250 250 STUDENTS
Download english protocol: DANAGENE SPIN SALIVADNA KIT INGLÉS
DANAGENE SPIN GENOMIC DNA KIT
This kit is designed for a efficient and fast extraction of genomic DNA from high quality from a wide variety of samples including blood, cultured cells, animal tissue, mouse tails, bacteria and yeast using for this MicroSpin columns with silica membranes which selectively bind DNA.
> Kit includes:
Tissues lysis buffer, lysis/binding buffer, proteinase K, disinhibition buffer, wash buffer, elution buffer, and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.
DANAGENE SPIN BLOOD DNA KIT
This kit is designed for a fast purification of pure genomic DNA from whole blood, serum, plasma, body fluids and “spots” dried blood using Microspin columns with silica membrane which selectively binds DNA. The kit uses a new formulated lysis/binding buffer specific to DNA extraction from blood samples.
> Kit includes:
RBC buffer, tissues lysis buffer, lysis/binding buffer, proteinase K, disinhibition buffer, wash buffer, elution buffer, and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.
DANAGENE FFPE DNA KIT
This kit is optimized for a fast method of DNA extraction from paraffin-embedded and formalin-fixed (FFPE) tissues. The process omits the use of flammable and malodorous xylene and d-limonene commonly used for deparaffinization, instead own deparaffinization buffer is used for the complete dissolution of paraffin to release the tissue.
> Kit includes:
Deparaffinization buffer, tissues lysis buffer, lysis/binding buffer, proteinase K, disinhibition buffer, wash buffer, elution buffer, and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.
DANAGENE SPIN FOOD-STOOL kit
This kit has been optimized for an efficient and fast purification of total DNA from :
1. Fresh/frozen stool samples.
2. Various food samples (raw material and processed food).
> Kit includes:
CTAB Buffer, binding buffer, proteinase K, desinhibition buffer, wash buffer, elution buffer and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.
BACTERIAL DNA EXTRACTION KIT
The objective of this practice is to introduce the principles of extracting chromosomal DNA from E. coli bacterial cells. Students learn the structure and function of nucleic acids containing a bacterium.
> Kit includes:
Bacterial pellets, lysis solution, proteins precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.
PLASMID DNA EXTRACTION KIT
The objective of this practice is to introduce the principles of extracting plasmid DNA from bacterial cells. Students will learn the structure and function of DNA plasmids.
> Kit includes:
Bacterial pellet, resuspension buffer, lysis buffer, neutralization/binding buffer, wash buffer, elution buffer and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge and 100% ethanol.
DANAGENE SALIVA RNA Kit
1.DANASALIVA RNA Sample Collection Kit provides a safe and rapid all-in-one procedure for the collection, stabilization and transportation of 1 ml saliva samples at ambient temperature that stabilizes RNA from the moment of collection for 1 month.
Saliva samples are collected by spitting inside the collection funnel which has been assembled with the collection tube. After collecting 1 ml saliva the content of saliva preservation solution are then added and mixed with the collected saliva. The saliva collection tube is sent to the laboratory for RNA isolation and analysis using the DANAGENE SALIVA RNA Kit.
2.DANAGENE SALIVA RNA Kit has been designed for a fast and efficient purification of total RNA from preserved saliva samples.
The process includes a cell lysis with proteinase K followed by a precipitation of the proteins and part of genomic DNA. Later, by a precipitation with isopropanol, total RNA is obtained, which is finally rehydrated. Finally, for removal of genomic DNA contamination is used an approach consisting of two sequential filtrations with different MiroSpin columns.
AUTOMATED NUCLEIC ACID EXTRACTION
ZiXpress-32® is a compact and reliable automated extraction platform based on Magnetic Pillar (Rod) technology, which can extract up to 32 samples in 20 to 40 minutes with the use of Zi-Beads® magnetic particles.
It is an open and flexible platform with CE certification that allows to operate with optimized kits of pre-loaded / non-pre-loaded reagents with the protocols already introduced, offering the final user the freedom to use it with the supplied consumables or with any other standard consumable, and customize their own protocols to precisely meet any application need.
