IMMUNOLOGY

INMUNOLOGÍA


4. IMMUNOLOGY

INTRODUCTION TO ELISA REACTION

Your students will learn the basic principles of the Enzyme-linked Immunosorbent Assay (ELISA) in this precise and sensitive antibody-based detection kit.

Experiment components do not contain human serum.

> Kit includes:
Antigens, primary and secondary antibodies, peroxide co-substrate, hydrogen peroxide, ABTS substrate, phosphate buffered saline, tubes, plates, and transfer pipettes. 
> You need:
Distilled water, 37º C incubation oven, automatic micropipettes with tips and laboratory glassware.

icons-ref-greenELISA1
      10 groups of students

imgcat--ELISA

SIMULATION OF HIV DETECTION BY ELISA

This experiment’s aim is to provide students the molecular biology and pathogenesis basics of acquired immunodeficiency syndrome (AIDS).

An HIV test detects HIV infection indirectly using an ELISA test against HIV antibodies in the blood. The test works by taking antibodies from the patient’s blood and adding them to a microtiter plate coated with HIV antigen. If HIV antibodies are present in the blood, they will bind to the antigens on the plate. This binding is detected with an enzyme-linked secondary antibody that causes a color change upon addition of substrate. In this experiment, students will perform an ELISA test by coating microtiter plate wells with simulated HIV antigen and then test simulated donor serum for anti-HIV antibodies.

> Kit includes:
Simulated HIV antigens, serum samples, antibodies, buffers, microtiter plates, assorted pipettes and microtest tubes. 
> You need:
37ºC incubation oven, micropipettes with tips, pipette pumps, laboratory glassware, and distilled water.

icons-ref-greenELISA2
     10 groups of students

imgcat--ELISAhvi

QUANTITATIVE ELISA

Antibodies are highly specific in their recognition of antigens. This ELISA experiment demonstrates the quantitation of varying concentrations of viral antigens as detected by the intensity of the color reaction due to the accumulation of products.

> Kit includes:
Antigens, primary and secondary antibodies, substrate solution, phosphate buffered saline, blocking agent, stop solution, tubes, plates, and transfer pipettes.
> You need:
Distilled water, 37º C incubation oven, micropipettes with tips, laboratory glassware.

icons-ref-greenELISA3
     6 groups of students

SIMULATION OF HIV DETECTION BY WESTERN BLOT

The second assay used to confirm a positive HIV ELISA result is the Western Blot. Students separate protein samples from hypothetical patients on agarose gels, transfer the samples to a membrane and detect the simulated HIV proteins. This kit is an introductory level experiment.

> Kit includes:
Samples, standard molecular weight markers, protein agarose, various buffers and reagents, PVDF membrane, filter paper, stain, 1 ml pipette, 100 ml graduated cylinder.
> You need:
Electrophoresis apparatus, power supply, micropipettes with tips, microwave, incubation oven, shaker platform, lab glassware, small plastic trays, microtest tubes, pipette pumps, metric rulers, distilled water, methanol, and glacial acetic acid.

icons-ref-greenELISA4
     6 groups of students

imgcat--WESTERN-BLOT

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