BIOTECHNOLOGY

BIOTECNOLOGÍA


2.4. MUTAGENICITY TESTS

THE DOSE MAKES THE POISON: TESTING THE ENVIRONMENTAL IMPACTS OF POLLUTION

Biological assays, or bioassays, are powerful tools that allow scientists to determine the effects of a given substance on living organism. In this inquiry-based lab students plan and perform a plant bioassay to determine the environmental Hazard of common point and non-point source pollutants. The results are analyzed using averages, standard deviations, and TC50 calculations, integrating STEM.

> Kit includes:
Radish seeds, plant growth medium, antifungal powder, pollutant solutions, cheese cloth, Petri dishes, transfer pipets, seriological pipet, conical tubes, microcentrifuge tubes and small loops.
> You need:
Watherbath, pipet pump, microwave or hot plate, 70% etanol, sterile wáter, bleach, automatic micropipet and tips, parafilm, string, rulers, growth light (optional) and analytical balance (optional).

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 10 groups of students

X PROJECT: WICH ONE IS THE MUTANT?

Gregor Mendel studied pea plants over the course of many years to understand inheritance. In this practice the students can use 3 different genetics strains of Arabidopsis Quick Plants™ to see the genetic ratios for themselves.

This practice requires several weeks for the plants growth.

> Kit includes:
Arabidopsis Quick Plant™ seeds, seed gel, peat pellets, growth containers, fertilizar, and magnifying glasses.
> You need:
Fluorescent plant growth lights (recommended), spray bottle for fertilizer, and distilled water.

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 10 groups of students
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