BIOTECHNOLOGY

BIOTECNOLOGÍA


2.4. MUTAGENICITY TESTS

TOXICITY DETECTION OF POLLUTANTS IN FRESHWATER

This experiment has been adapted from a freshwater quality test which uses Daphnia magna to determine toxicity levels of freshwater. A simulated “toxicant” is provided to simulate environmental pollution in freshwater lakes, rivers and streams.

Hydrolysis of a fluorescent substrate by Daphnia is used to determine the level of toxicants. Results are observed by using long wave ultraviolet light. Calculations for lethal concentration are determined.

> Kit includes:
Fluorescent detection substrate, simulated toxicant concentrate, toxicity reduction reagent, 1 molded exposure chamber, wide bore transfer pipettes, and calibrated plastic pipettes.
> You need:
Daphnia magna, 4 additional molded exposure chambers (Ref. ED-965), long wave UV light, white light visualization system, microscope, large glass vessel, beakers, UV protective goggles, spring water, test tubes, 5 ml pipettes and pumps.

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 5 groups of students

X PROJECT: WICH ONE IS THE MUTANT?

Gregor Mendel studied pea plants over the course of many years to understand inheritance. In this practice the students can use 3 different genetics strains of Arabidopsis Quick Plants™ to see the genetic ratios for themselves.

This practice requires several weeks for the plants growth.

> Kit includes:
Arabidopsis Quick Plant™ seeds, seed gel, peat pellets, growth containers, fertilizar, and magnifying glasses.
> You need:
Fluorescent plant growth lights (recommended), spray bottle for fertilizer, and distilled water.

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 10 groups of students
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