MOLECULAR BIOLOGY

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1.7. NUCLEIC ACID SEQUENCING

It’s the process to determining the nucleotide sequence comprising a DNA or RNA molecule and which is represented by the initials of the nitrogenous bases they carry.

SEQUENCING THE HUMAN GENOME

Actual data representing important genes from automated DNA Sequencers are provided. Students will determine the DNA sequence, compare and extrapolate database information and identify the gene product and other closely related proteins.

> Kit includes:
Automated sequencing printouts.
> You need:
Computer access to the internet.

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       10 groups of students


 Download protocol 

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ADN-BIOINFORMATICS

DNA sequence information is being compiled by various genome initiatives and numerous research groups around the world. The management of this data is known as bioinformatics. This information is stored in various DNA sequence databases which can be readily accessed via the internet.

In this experiment, students read autoradiographs containing DNA sequences which represent segments of important cellular genes. Using bioinformatics databases, students compare and extrapolate database information and identify the gene product.

> Kit includes:
3 sets of 4 autoradiograms.
> You need:
White light visualization system, computer access to the internet.

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  12 groups of students

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