MOLECULAR BIOLOGY

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1.1. EXTRACTION AND PURIFICATION OF
NUCLEICS ACIDS

icono_doctor BASIC LEVEL

DANAEXTRACTOR KIT

This practice is an introduction to molecular biology. This kit provides basic DNA extractions from animal tissues (chicken liver) and vegetables.

The kit includes everything you need so that each student can carry out an individual practice, in the classroom itself.

> Any additional equipment or material is not required.

DANAEXTRACTOR KIT

icono_doctor INTERMEDIATE LEVEL

DANAEXTRACTOR SALIVA 2 Kit

This kit allows students make an extraction of their own DNA quickly and easily from a small sample of his saliva, a laboratory microcentrifuge is not needed.

To carry out this practice the laboratory has to have 20-200 microliters micropipettes and 100-1000 microliters micropipettes and their respective tips. This material also can be supplied by BIOTED.

The remaining material necessary to carry out the practice by each student individually is included in the kit.

The DNA appears as a strand in the solution that the student could take home.

icon-refDNES2-25           25 students

icono_doctor ADVANCED LEVEL

The extraction process involves cell lysis to release nucleic acids and the inactivation of cellular nucleases (DNases and RNases) to prevent degradation of nucleic acids.

The purification process is the separation of the nucleic acids from the other components.

There are different techniques for purification:

A. SALTING-OUT

Proteins precipitation with high salt concentrations.

DANAGENE SALIVA KIT

This kit allows you to isolate DNA from a saliva sample of students to obtain a quality DNA for use in PCR.

> Kit includes:
Lysis solution, protein precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, isopropanol and 70% ethanol.

icon-ref0603.4           50 students
icon-ref0603.41        160 students

DANAGENE SALIVA KIT-2

DANAGENE BLOOD DNA KIT

This kit allows you to isolate DNA from a blood sample to obtain a quality DNA for use in PCR.

> Kit includes:
RBC solution, lysis solution, proteins precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, isopropanol and 70% ethanol.

icon-ref0601           100 ml/blood
icon-ref0602           200 ml/blood

DANAGENE BLOOD DNA KIT-2

DANAGENE PLANT DNA KIT

This kit allows you to isolate DNA from different plants samples to obtain a quality DNA for use in PCR.

> Kit includes:
Extraction buffer, lysis solution, RNAse, PVP solution, protein precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, isopropanol and 70% ethanol.

icon-ref0604.1           50 extractions
icon-ref0604.2      200 extractions

DANAGENE PLANT DNA KIT-2

B. ADSORPTION CHROMATOGRAPHY.

It is based on the adsorption capacity of nucleic acids to the silica membrane in the presence of chaotropic salts which come in small columns. We have a wide range of kits that use columns, you can visit at www.danagen.es.

We propose the following kits at educational level:

DANAGENE SPIN GENOMIC DNA KIT

This kit is designed for a efficient and fast extraction of genomic DNA from high quality from a wide variety of samples including blood, cultured cells, animal tissue, mouse tails, bacteria and yeast using for this MicroSpin columns with silica membranes which selectively bind DNA.

> Kit includes:
Tissues lysis buffer, lysis/binding buffer, proteinase K, disinhibition buffer, wash buffer, elution buffer, and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.

icon-ref0605.1           50 extractions
icon-ref0605.2      250 extractions

DANAGENE SPIN BLOOD DNA KIT

This kit is designed for a fast purification of pure genomic DNA from whole blood, serum, plasma, body fluids and “spots” dried blood using Microspin columns with silica membrane which selectively binds DNA. The kit uses a new formulated lysis/binding buffer specific to DNA extraction from blood samples.

> Kit includes:
RBC buffer, tissues lysis buffer, lysis/binding buffer, proteinase K, disinhibition buffer, wash buffer, elution buffer, and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.

icon-ref0606.1           50 extractions
icon-ref0606.2       250 extractions

DANAGENE FFPE DNA KIT

This kit is optimized for a fast method of DNA extraction from paraffin-embedded and formalin-fixed (FFPE) tissues. The process omits the use of flammable and malodorous xylene and d-limonene commonly used for deparaffinization, instead own deparaffinization buffer is used for the complete dissolution of paraffin to release the tissue.

> Kit includes:
Deparaffinization buffer, tissues lysis buffer, lysis/binding buffer, proteinase K, disinhibition buffer, wash buffer, elution buffer, and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.

icon-ref0610.1           50 extractions

BACTERIAL DNA EXTRACTION KIT

The objective of this practice is to introduce the principles of extracting chromosomal DNA from E. coli bacterial cells. Students learn the structure and function of nucleic acids containing a bacterium.

> Kit includes:
Bacterial pellets, lysis solution, proteins precipitation solution and DNA hydration solution.
> You need:
Micropipettes, microtubes, microcentrifuge, incubation bath, isopropanol and 100% ethanol.

icon-refADNBACT      25 extractions

EXTRACCIÓN ADN BACTERIANO KIT-2

PLASMID DNA EXTRACTION KIT

The objective of this practice is to introduce the principles of extracting plasmid DNA from bacterial cells. Students will learn the structure and function of DNA plasmids.

> Kit includes:
Bacterial pellet, resuspension buffer, lysis buffer, neutralization/binding buffer, wash buffer, elution buffer and microcolumns.
> You need:
Micropipettes, microtubes, microcentrifuge and 100% ethanol.

icon-refADNPLASMIDO25 extractions

EXTRACCIÓN ADN PLASMIDICO KIT-2

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